Genetic Predisposition

The NCT/UCC Research Group “Genetic Predisposition” focuses on the identification of disease causing germline variants/alterations in children with cancer with a special focus on acute leukemia of childhood, as well as the elucidation of the oncogenic capacity of these genetic variants in regard to environmental exposures.

Methodology

• Trio-sequencing of child-parent trios in children newly diagnosed with cancer
• Analysis of the sequencing data for known classical cancer predispositions and VUS
• Transfection, Transduction, Knockdown and Overexpression in cell systems
• CRISPR-Cas9 based technologies
• Protein biochemical methods (Western Blot, Immunoprecipitation, Reporter gene assays)
• Sequencing methods (NGS, Sanger-sequencing, Deep-Sequencing) (in cooperation)
• Transgenic mouse models