Department of Functional Imaging in Surgical Oncology

Our research is dedicated to the development of excellent techniques for biomedical imaging. The advancement of new targeted contrast agents and novel imaging modalities will pave the way for personalized therapy and high precision treatments in the near future. Imaging in the short-wave infrared region (SWIR) is a new technology for biomedical applications. It provides several advantages over the visible and near-infrared regions: general lack of autofluorescence, low light absorption by blood and tissue, and reduced scattering. In this wavelength range tissues become translucent. Recent progress in detection technology and the development of probes demonstrated that, in principal, SWIR imaging enables applications which were previously not feasible with any other technique. These advantages will enable new capabilities in preclinical and clinical imaging. 

Our aims

• We develop near-infrared (NIR) and shortwave-infared (SWIR) imaging to provide solutions for guiding decision-making in surgical oncology.
• We develop contrast agents for NIR and SWIR fluorescence-guided surgery, targeting identification of residual cancer and risk structures such as nerves.
• We develop label-free imaging methods for identifying lymph nodes, fat, and other tissue components.
• We perform basic research on NIR and SWIR imaging and microscopy, focusing on methods to improve sensitivity, imaging speed and depth of detection.

 

 

 

 

 

 

 

 

 

 

 

 

Tjadina Klein

Ph. D. student

Email: tjadina-wencke.klein(at)nct-dresden.de

Alumni

• Samuel Adler
• Ilaria Balba
• Thomas Bischof
• Berit Blume
• Nina Buettner
• Emily Cosco
• Asli Ergin
• Gina Fürtjes
• Xhenifer Guza
• Sofia Kieffa
• Jakob Lingg
• Bunyamin Pekdemir
• Shyam Ramakrishnan
• Hannes Rolbieski
• Mara Saccomano
• Katharina Tartler
• Peter Tsrunchev
• Martin Warmer
• Joycelyn Yiu

1. In vivo NIR-II fluorescence imaging for biology and medicine.
Feifei Wang, Yeteng Zhong, Oliver T. Bruns, Yongye Liang, Hongjie Dai
Published: 04 March 2024; Nature Photonics (2024); Link to the article

2. Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time.
Cosco ED, Spearman AL, Ramakrishnan S, Lingg JGP, Saccomano M, Pengshung M, Arús BA, Wong KCY, Glasl S, Ntziachristos V, Warmer M, McLaughlin RR, Bruns OT*, Sletten EM*. Nat Chem. 2020 Dec;12(12):1123-1130. doi: 10.1038/s41557-020-00554-5. Epub 2020 Oct 19. PMID: 33077925 DOI: 10.1038/s41557-020-00554-5

3. Targeted multicolor in vivo imaging over 1,000 nm enabled by nonamethine cyanines.
Bandi VG*, Luciano MP*, Saccomano M*, Patel NL, Bischof TS, Lingg JGP, Tsrunchev PT, Nix MN, Ruehle B, Sanders C, Riffle L, Robinson CM, Difilippantonio S, Kalen JD, Resch-Genger U, Ivanic J, Bruns OT*, Schnermann MJ*. Nat Methods. 2022 Mar;19(3):353-358. doi: 10.1038/s41592-022-01394-6. Epub 2022 Feb 28. PMID: 35228725 DOI: 10.1038/s41592-022-01394-6

4. Next-generation in vivo optical imaging with short-wave infrared quantum dots.
Bruns OT*, Bischof TS*, Harris DK, Franke D, Shi Y, Riedemann L, Bartelt A, Jaworski FB, Carr JA, Rowlands CJ, Wilson MWB, Chen O, Wei H, Hwang GW, Montana DM, Coropceanu I, Achorn OB, Kloepper J, Heeren J, So PTC, Fukumura D, Jensen KF, Jain RK, Bawendi MG. Nat Biomed Eng. 2017;1:0056. doi: 10.1038/s41551-017-0056. Epub 2017 Apr 10. PMID: 29119058 DOI: 10.1038/s41551-017-0056​​​​​​​

5. Bright Chromenylium Polymethine Dyes Enable Fast, Four-Color In Vivo Imaging with Shortwave Infrared Detection.
Cosco ED, Arús BA, Spearman AL, Atallah TL, Lim I, Leland OS, Caram JR, Bischof TS, Bruns OT*, Sletten EM*. J Am Chem Soc. 2021 May 12;143(18):6836-6846. doi: 10.1021/jacs.0c11599. Epub 2021 May 3. PMID: 33939921 DOI: 10.1021/jacs.0c11599

6. High-Resolution Multicolor Shortwave Infrared Dynamic In Vivo Imaging with Chromenylium Nonamethine Dyes.
Anthony L. Spearman, Eric Y. Lin, Emily B. Mobley, Andriy Chmyrov, Bernardo A. Arús, Daniel W. Turner, Cesar A. Garcia, Kyle Bui, Christopher Rowlands, Oliver T. Bruns, Ellen M. Sletten. J Am Chem Soc. 2025. doi: 10.1021/jacs.5c03673

7. Shortwave-Infrared Line-Scan Confocal Microscope for Deep Tissue Imaging in Intact Organs.
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arús, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov. Laser & Photonics Reviews. 2023. DOI: 10.1002/lpor.202300292

8. Development of a shortwave infrared sinuscope for the detection of cerebrospinal fluid leaks.
Klein TW, Yang S, Tusty MA, Nayak JV, Chang MT, Bruns OT*, Bischof TS*, Valdez TA*. J Biomed Opt. 2023 Sep;28(9):094803. doi: 10.1117/1.JBO.28.9.094803. Epub 2023 May 12. PMID: 37188003 DOI: 10.1117/1.JBO.28.9.094803

9. Shortwave infrared fluorescence imaging of peripheral organs in awake and freely moving mice.
Arús BA, Cosco ED, Yiu J, Balba I, Bischof TS, Sletten EM, Bruns OT. 
Front Neurosci. 2023 May 18;17:1135494. doi: 10.3389/fnins.2023.1135494. eCollection 2023.
PMID: 37274204 DOI: 10.3389/fnins.2023.1135494

10. Preventing cation intermixing enables 50% quantum yield in sub-15 nm short-wave infrared-emitting rare-earth based core-shell nanocrystals.
Arteaga Cardona F, Jain N, Popescu R, Busko D, Madirov E, Arús BA, Gerthsen D, De Backer A, Bals S, Bruns OT, Chmyrov A*, Van Aert S*, Richards BS*, Hudry D*. Nat Commun. 2023 Jul 25;14(1):4462. doi: 10.1038/s41467-023-40031-4. PMID: 37491427 DOI: 10.1038/s41467-023-40031-4

11. Dramatic Impact of Materials Combinations on the Chemical Organization of Core–Shell Nanocrystals: Boosting the Tm3+ Emission above 1600 nm.
Fernando Arteaga Cardona, Eduard Madirov, Radian Popescu, Di Wang, Dmitry Busko, Dominique Ectors, Christian Kübel, Yolita M. Eggeler, Bernardo A. Arús, Andriy Chmyrov, Oliver T. Bruns, Bryce S. Richards*, Damien Hudry* Published: 12 September 2024; ACS Nano (2024); Link to the article

12. Intraoperative microscopic autofluorescence detection and characterization in brain tumors using stimulated Raman histology and two-photon fluorescence. 
Fürtjes G, Reinecke D, von Spreckelsen N, Meißner A-K, Rueß D, Timmer M, Freudiger C, Ion-Margineanu A, Khalid F, Watrinet K, Mawrin C, Chmyrov A, Goldbrunner R, Bruns OT, Neuschmelting V. Frontiers in Oncology. 2023. DOI: 10.3389/fonc.2023.1146031

13. Absorption by water increases fluorescence image contrast of biological tissue in the shortwave infrared.
Carr JA, Aellen M, Franke D, So PTC, Bruns OT*, Bawendi MG*. Proc Natl Acad Sci U S A. 2018 Sep 11;115(37):9080-9085. doi: 10.1073/pnas.1803210115. Epub 2018 Aug 27. PMID: 30150372  DOI: 10.1073/pnas.1803210115

14. Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green.
Carr JA, Franke D, Caram JR, Perkinson CF, Saif M, Askoxylakis V, Datta M, Fukumura D, Jain RK, Bawendi MG*, Bruns OT*. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):4465-4470. doi: 10.1073/pnas.1718917115. Epub 2018 Apr 6. PMID: 29626132 DOI: 10.1073/pnas.1718917115

15. Nanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning.
Luo J, Molbay M, Chen Y, Horvath I, Kadletz K, Kick B, Zhao S, AlMaskari R, Singh I, Ali M, Bhatia HS, Minde DP, Negwer M, Hoeher L, Calandra GM, Groschup B, Su J, Kimna C, Rong Z, Galensowske N, Todorov MI, Jeridi D, Ohn TL, Roth S, Simats A, Singh V, Khalin I, Pan C, Arús BA, Bruns OT, Zeidler R, Liesz A, Protzer U, Plesnila N, Ussar S, Hellal F, Paetzold J, Elsner M, Dietz H, Erturk A. Nature Biotechnology (2025);  Link to the article

Selected pre-prints

1. Park RK*, Arús BA*, Lee JY, Weitzenberg MM, Lee MC, Nyaeme MS, Barthel J, Balsamo G, Baik FM, Speirs K, Blume B, Heller-Algazi M, Chmyrov A, Plettenburg O, Megwalu UC, Weitz J, Distler M, Bruns OT*, Valdez TA*. Intraoperative nerve imaging with sodium fluorescein. medRxiv, https://doi.org/10.1101/2025.02.08.25321923

2. Arús BA, Yiu J, Lingg JGP, Hofmann A, Fumo AR, Ji H, Jethwa C, Park R K, Henderson J,  Mishra K, Mukha I, Stiel AC, Santovito D, Weber C, Reeps C, Rohm M, Bartelt A, Valdez TA, Chmyrov A, Bruns OT. Macroscopic label-free biomedical imaging with shortwave infrared Raman scattering. bioRxiv, 10597863, 2024. https://doi.org/10.1101/2024.06.10.597863

3.  Xu C*, Liu Y*, Arús BA*, Mishra K*, Luciano M, Bandi V, Kumar A, Guo Z, Bick M, Xu M, Zhang K, Lingg J, Bae J, Kang A, Gerben SR, Bera AK, Vaughan JC, Manton JD, Derivery E, Schnermann MJ, Stiel AC, Bruns OT, Baker D. De novo design of near infrared fluorescent proteins. Research Square, 4652998, 2024. https://doi.org/10.21203/rs.3.rs-4652998/v1

* means either shared first or shared last authorship

Full publication list available here

• Chan Zuckerberg Initiative
• EKFZ
• Horizon Europe
• Helmholtz Imaging Project
• BMBF - Better View
• SFB1123
• Alexander von Humboldt-Stiftung
• Stiftung Deutsche Krebshilfe
• Richard und Annemarie Wolf foundation
• National Institute of Health (NIH)

Prof. Dr. Ellen M. Sletten >>
Department of Chemistry and Biochemistry, University of California, Los Angeles, California, USA

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Dr. Martin J. Schnermann >>
National Cancer Institute, National Institute of Health, Bethesda, Maryland, USA

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Dr. Christopher Rowlands >>
Faculty of Engineering, Department of Bioengineering, Imperial College, London, UK

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Prof. Dr. Tulio A Valdez >>
Stanford University, Stanford, California, USA

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Prof. Dr. Oliver Plettenburg >>
Institute of Organic Chemistry, Leibniz University Hannover, Germany

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Dr. André C. Stiel >>
Cell Engineering Group, Helmholtz Munich, Germany

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Dr. Volker Neuschmelting >>
University Hospital Cologne, Germany

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Prof. Dr. Alexander Bartelt >>
Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Germany

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Dr. Damien Hudry >>
Institute of Microstructure Technology (IMT), Karlsruhe Institute of Technology, Germany

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Prof. Dr. Bryce S. Richards >>
Institute of Microstructure Technology (IMT), Karlsruhe Institute of Technology, Germany

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Dr. rer. nat. Ute Resch-Genger >>
Department of Biophotonics, Bundesanstalt für Materialforschung und -prüfung, Berlin, Germany

• Perkin Elmer Lambda 1050+, including Three Detectors module and the 150mm Integrating Sphere module
• Home-built photoexcitation/photoluminescence spectrometer based on an NKT SuperK and a Princeton Instruments spectrograph with a Pixis and Pylon. Excitation 400-1600nm, detection 400-1600nm

Are you interested in joining our team? You can find some of the current positions by following the respective link below. But we are always looking for new team members from different backgrounds to join our team. Feel free to approach us about Master thesis projects, PhD projects and Postdoc positions.

For more information or if you like to send an initiative application, please contact Ms. Maria Voigt by email to: maria.voigt(at)nct-dresden.de

Please attach a motivation letter describing why you want to join our group and which specific project you are interested in as well as your CV to your email.

Contribution in the edition 25/01 of Laborjournal

Link to the article with Oliver Bruns: 'Blick mit Infrarotlicht mitten ins Leben - Live Cell Imaging'
(by Mario Rembold)

Full edition of the journal 25/01 with the article can be found here

Helmholtz High Impact Award for Oliver Bruns and Ellen Sletten

Please also see the media reviews for further information:
TU Dresden
Helmholtz Society
Stifterverband
Helmholtz Imaging (Interview)

Press release on funding by Chan Zuckerberg Initiative
We once again received renowned funding from the Chan Zuckerberg Initiative. The link to the detailed press release can be found here.

 

BDC Surgeon Talk 

with Oliver Bruns 'Molekulare Bildgebung - Zukunftmusik oder schon Realität?' (German only)

This and other podcasts can be found here (link to external page).

Introducing Oliver Bruns. TU Dresden

Prof. Oliver Bruns - University-Wide Inaugural Lecture

FBOO @ WMIC Prague

FBOO @ chemTUgether at TU Dresden